• br Acknowledgments We would like to thank

  2018-10-20

  

  Acknowledgments We would like to thank Dr. Keisuke Okita and Prof. Shinya Yamanaka for providing the plasmids for reprogramming and Dr. Feng Zhang for providing the plasmid for gene editing. We thank Ida Jørring and Bente Smith Thorup for excellent technical assistance in the cell culture. We tha

• To derive hiPSC from patient fibroblasts we adopted

  2018-10-20

  

  To derive hiPSC from patient fibroblasts, we adopted previous protocols (Okita et al., 2011; Rasmussen et al., 2014) and electroporated the patient\'s skin fibroblasts with episomal plasmids expressing human OCT4, SOX2, L-MYC, KLF4, NANOG, LIN28, and short hairpin RNA against TP53. To demonstrate th

• melatonin receptor agonists br Materials and methods br Auth

  2018-10-20

  

  Materials and methods Author disclosure statement Acknowledgments This study was supported by fund from National Medical Research Council (NMRC) of Singapore, the Translational Clinical Research (TCR) award (NMRC/TCR/013-NNI/2014) and STaR award (NMRC/STaR/014/2013). We thank Ms. Fiona Seti

• Thus transplanted iNSCs inhibit NF

  2018-10-20

  

  Thus, transplanted iNSCs inhibit NF-κB activation in the ampk inhibitors post-CHI. In brief, these results indicate that iNSC grafts may act through NF-κB signaling to regulate the immune response. Here are the details: 1) the levels of IL-1β and TNF-α secreted by immune cells are down-regulated as

• Here we present an algorithm KeyGenes that we

  2018-10-20

  

  Here we present an algorithm, KeyGenes, that we have used on NGS data extracted from tissues of 21 different human fetal organs, both embryonic and extraembryonic (plus the maternal endometrium), from the first and second trimesters of development to determine a panel of classifier genes that would

• We subsequently tested the functional consequences of this f

  2018-10-20

  

  We subsequently tested the functional consequences of this fibrogenic plasticity in dystrophic muscle. The fluorescence-activated cell sorting (FACS)-isolated ionomycin of endothelial and myogenic origins (YFP+) that had reduced (or lost) expression of CD31 or α7-INTEGRIN (YFP+CD31low/− or YFP+α7lo

• Understanding http www apexbt com media

  2018-10-20

  

  Understanding the regulatory pathways that control the establishment of the different hematopoietic programs and developing methods to identify and isolate progenitors for each are essential to accurately model hematopoiesis in ESC and induced PSC differentiation cultures and ultimately to generate

• Introduction Human pluripotent stem cells hPSCs

  2018-10-20

  

  Introduction Human pluripotent stem ras inhibitor (hPSCs), i.e., human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), can give rise to functional neurons (reviewed in Gage and Temple, 2013). Human peripheral neurons are difficult to differentiate, but a recently pu

• Although we followed all of the

  2018-10-20

  

  Although we followed all of the rules, we hesitate to use the phrase cGMP-compliant because this is a determination that will ultimately be made by the regulatory authorities when the entire IND package is provided to the FDA. We also note that the approval of the use of this line as part of an IND

• Avasimibe Here we addressed these problems by focusing on co

  2018-10-20

  

  Here we addressed these problems by focusing on control of the regional identity of PSC-derived neural progenitors and neurons. The developing neural tube is subdivided into distinct regions along the anteroposterior (A-P) and dorsoventral (D-V) axes, and each region produces a specific subtype of n

• Nishi et al a b have developed a method of

  2018-10-20

  

  Nishi et al. (2014a, 2014b) have developed a method of inducing cancer stem-like na+/ca2+ exchanger (CSCs) through the reprogramming and partial differentiation of the immortalized but otherwise normal MCF10A human mammary epithelial cell line. To experimentally test our computational model of onco

• It has previously been reported that differentiation of hESC

  2018-10-20

  

  It has previously been reported that differentiation of hESCs without the embryonic body stage led to an increase in the capacity of hESCs to differentiate into osteogenic progenitor mct2 inhibitor (Karp et al., 2006). Consistent with these findings, our differentiation culture method resulted in ce

• Since hiPSC CMs can mature in vitro over time

  2018-10-20

  

  Since hiPSC-CMs can mature in vitro over time in culture (Lundy et al., 2013), they may prove valuable for investigating the developmental time course of early-stage cardiac diseases, representing a unique platform to predict adverse prenatal outcomes and to develop therapies. We reported FCM hiPSC-

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments B.Z. was supported by an NYSTEM post-doctoral training fellowship, The SKI Stem Cell Research Facility is supported by NYSTEM grants C029153 and C024175 and The Starr Foundation. The work was further supported in part by NIH/NCI g

• The importance of the interaction between fibroblasts and CM

  2018-10-20

  

  The importance of the interaction between fibroblasts and CMs in cardiac hypertrophy has been previously demonstrated. In neonatal rat and mouse CMs, hypertrophy was dependent on exposure to fibroblasts or their conditioned media, and was mediated by Vadimezan paracrine TGFβ1 release (Fredj et al.,

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